Function of lymphocyte subpopulations in chronic lymphocytic leukemia. Activity in the allogeneic and autologous mixed lymphocyte reaction

We have defined two subpopulations of B lymphocytes in chronic lymphocytic leukemia (CLL). The major variant (termed typical) was characterized by the presence of a relatively homogeneous population of small-to-medium-sized lymphocytes with low-density SmIgM and no evidence of intracellular Ig synthesis. A minor group (termed atypical) was identified by the presence of a pleomorphic cell population with few small lymphocytes. The predominant SmIg was IgG, which was detected intracellularly as well as in the serum. The atypical group appeared to be an arrest at a later stage of differentiation where a switch from IgM to IgG synthesis and secretion had occurred. Clinical correlation suggested several clear-cut differences in clinicopathologic characteristics, but the median survivals for both groups at 2 yr was nearly identical.

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T-lymphocyte subpopulations in chronic lymphocytic leukemia: A quantitative and functional study

Cancer ◽

10.1002/1097-0142(19811115)48:10<2191::aid-cncr2820481013>3.0.co;2-9 ◽

1981 ◽

Vol 48 (10) ◽

pp. 2191-2197 ◽

Cited By ~ 27

Author(s):

G. Semenzato ◽

A. Pezzutto ◽

C. Agostini ◽

M. Albertin ◽

G. Gasparotto

Keyword(s):

Chronic Lymphocytic Leukemia ◽

T Lymphocyte ◽

Lymphocyte Subpopulations ◽

Lymphocytic Leukemia ◽

Functional Study ◽

T Lymphocyte Subpopulations

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Heterogeneity of the response of chronic lymphocytic leukemia cells to phorbol ester

Blood ◽

10.1182/blood.v60.5.1082.1082 ◽

1982 ◽

Vol 60 (5) ◽

pp. 1082-1088 ◽

Cited By ~ 20

Author(s):

J Okamura ◽

EW Gelfand ◽

M Letarte

Keyword(s):

Chronic Lymphocytic Leukemia ◽

Phorbol Ester ◽

Mixed Lymphocyte Reaction ◽

Lymphocytic Leukemia ◽

Leukemic Cells ◽

Leukemia Cells ◽

Phenotypic Markers ◽

Tumor Promotor ◽

Bright Surface

Abstract The ability of the tumor promotor 12–0-tetradecanoylphorbol 13-acetate (TPA) to induce differentiation of leukemic cells was studied in 10 cases of chronic lymphocytic leukemia (CLL). An increase in modal volume and an enhancement of the capacity of te leukemic cells to stimulate in mixed lymphocyte reaction (MLR) was seen in the majority of cases. A significant increase in Ia expression was observed upon culture of leukemic cells with TPA in 6 of the 10 cases; 5 of these cases also showed an induction of cytoplasmic IgM production. Correlations between the phenotypic markers of the leukemic cells and their ability to respond to TPA were evaluated. CLL cells with low amounts to surface Ig. a volume less than or equal to 165 fl. and relatively low la expression responded well to TPA. Cells with bright surface Ig. a volume greater than or equal to 178 fl. and elevated amounts of Ia responded poorly to TPA. These results suggest that differences in the response of B leukemic cells to TPA reflect the underlying heterogeneity of the leukemic cells and might be correlated with their stage of maturation.

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Lactic dehydrogenase in normal and leukemia lymphocyte subpopulations: evidence for the presence of abnormal T cells and B cells in chronic lymphocytic leukemia

Blood ◽

10.1182/blood.v57.2.324.324 ◽

1981 ◽

Vol 57 (2) ◽

pp. 324-327 ◽

Cited By ~ 14

Author(s):

P Rambotti ◽

S Davis

Keyword(s):

T Cells ◽

B Cells ◽

Chronic Lymphocytic Leukemia ◽

T Lymphocytes ◽

B Lymphocytes ◽

Lactic Dehydrogenase ◽

Isozyme Pattern ◽

Lymphocyte Subpopulations ◽

Lymphocytic Leukemia ◽

Ldh Activity

Abstract Lactic dehydrogenase (LDH) was quantitated and the isozyme pattern studied in lymphocyte subpopulations from normal people and patients with chronic lymphocytic leukemia (CLL). Normal T lymphocytes differed from normal B lymphocytes in having greater total LDH activity (597.2 versus 252.1). Total LDH activity in CLL T cells (347.1) was lower than normal T cells., but not significantly different than normal B cells. Total LDH activity in CLL B cells (124.6) was lower then normal B cells and normal T cells. The isozyme pattern of normal T lymphocytes showed a higher activity in the LDH-1 band (26.7% versus 5.4%) but showed a lower activity in LDH-5 band (4.3% versus 16.3%) compared to normal B cells. Chronic lymphocytic leukemia T cells could be distinguished from CLL B cells by a high LDH-5 band (22.3% versus 7.6%) and from normal T cells by a high LDH-5 band (22.3% versus 4.3%) and a low LDH-1 band (7.3% versus 26.7%). CLL B cells could be distinguished from normal B cells by a low LDH-5 band (7.6% versus 16.3%). Thus, the LDH isozyme pattern distinguishes normal T lymphocytes from normal B lymphocytes, and normal T and B lymphocytes from CLL T and B lymphocytes.

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B lymphocyte subpopulations in chronic lymphocytic leukemia

Blood ◽

10.1182/blood.v47.2.229.229 ◽

1976 ◽

Vol 47 (2) ◽

pp. 229-235 ◽

Cited By ~ 23

Author(s):

RA Rudders

Keyword(s):

Chronic Lymphocytic Leukemia ◽

B Lymphocyte ◽

Lymphocyte Subpopulations ◽

Lymphocytic Leukemia ◽

Clinicopathologic Characteristics ◽

Homogeneous Population ◽

Clear Cut ◽

Igg Synthesis ◽

A Minor ◽

Ig Synthesis

Abstract We have defined two subpopulations of B lymphocytes in chronic lymphocytic leukemia (CLL). The major variant (termed typical) was characterized by the presence of a relatively homogeneous population of small-to-medium-sized lymphocytes with low-density SmIgM and no evidence of intracellular Ig synthesis. A minor group (termed atypical) was identified by the presence of a pleomorphic cell population with few small lymphocytes. The predominant SmIg was IgG, which was detected intracellularly as well as in the serum. The atypical group appeared to be an arrest at a later stage of differentiation where a switch from IgM to IgG synthesis and secretion had occurred. Clinical correlation suggested several clear-cut differences in clinicopathologic characteristics, but the median survivals for both groups at 2 yr was nearly identical.

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